https://www.picelkem.com/blog daily 0.75 2026-04-23 https://www.picelkem.com/blog/concept-to-data-beginner-friendly-guide-to-starting-your-first-biologic-investigation-ygtb6 monthly 0.5 2026-04-23 https://images.squarespace-cdn.com/content/v1/6698031d5baeb54365a9a728/824f68d6-ae52-411a-80a5-8ecb3c7fa964/1.JPG Blog - Alternative Two Step Purification of a Soluble Receptor Fc Fusion Biologic Using Mixed-Mode Chromatography  - Make it stand out Fig. 1 Mab Select Sure ™ Protein A Chromatogrpahy (Cytiva) https://images.squarespace-cdn.com/content/v1/6698031d5baeb54365a9a728/cb376a91-408d-4800-b5f7-584ab9073dcf/2.JPG Blog - Alternative Two Step Purification of a Soluble Receptor Fc Fusion Biologic Using Mixed-Mode Chromatography  - Make it stand out Fig. 2 Capture of CM on Nuvia wPrime 2A Mixed Modal Media The EGFR:Fc fusion protein was successfully captured on Nuvia™ wPrime2A in 20 mM NaPO₄ (pH 6.8), with this buffer concentration providing enhanced pH stability. Elution fractions were analyzed by reducing gel electrophoresis, revealing a capture purity of 90%. https://images.squarespace-cdn.com/content/v1/6698031d5baeb54365a9a728/155f9ca6-ed35-4dfd-9d61-a147ab266161/f3.JPG Blog - Alternative Two Step Purification of a Soluble Receptor Fc Fusion Biologic Using Mixed-Mode Chromatography  - Make it stand out Fig. 3 Polishing on Nuvia aPrime 4a post Nuvia wPrime 2a. The aggregate was reduced below 2% in 30m.M Tris, pH 7.8 with a 75mM wash step. Yields post wPrime yielded ~70% product and ~83% post protein A. Note that there is a shoulder peak which was determined to be non-proteinatious, possible chromaphore from the cell media. https://images.squarespace-cdn.com/content/v1/6698031d5baeb54365a9a728/9450a70e-8e92-4e92-9d86-f07ff75e1041/f4.JPG Blog - Alternative Two Step Purification of a Soluble Receptor Fc Fusion Biologic Using Mixed-Mode Chromatography  - Make it stand out Fig. 4 Nuvia HPQ post wPrime Capture. Nuvia aPrime elution dil 1:5 in buffer A; Conductivity below 10mS. https://images.squarespace-cdn.com/content/v1/6698031d5baeb54365a9a728/21ee951b-8165-4afb-a33f-cf03de44c0fc/t1.JPG Blog - Alternative Two Step Purification of a Soluble Receptor Fc Fusion Biologic Using Mixed-Mode Chromatography  - Make it stand out Table 1 Results of Polishing post protein A or wPrime Capture on a 1mL Forsight ™ Column. The condition which met our immediate interests was the HPQ media. https://images.squarespace-cdn.com/content/v1/6698031d5baeb54365a9a728/99f44930-54ba-4331-b4ad-b13ef60d7d12/f5.JPG Blog - Alternative Two Step Purification of a Soluble Receptor Fc Fusion Biologic Using Mixed-Mode Chromatography  - Make it stand out Fig. 5 The EGFR:Fc fusion protein was successfully captured on Nuvia™ wPrime2A in 20 mM NaPO₄ (pH 6.8), with this buffer concentration providing enhanced pH stability. Elution fractions were analyzed by reducing gel electrophoresis, revealing a capture purity of 90%—notable for a non-affinity medium. Additional bands at 34 and 28 kDa were observed, with Western analysis used to determine their relation to the product. Residual DNA and host cell protein (HCP) levels were also assessed. https://images.squarespace-cdn.com/content/v1/6698031d5baeb54365a9a728/62ee14cd-2fe1-40c2-8d10-6366c844c8f8/f6.JPG Blog - Alternative Two Step Purification of a Soluble Receptor Fc Fusion Biologic Using Mixed-Mode Chromatography  - Make it stand out Fig. 6 Polishing on Nuvia™ HPQ resulted in purity exceeding 98% by HPLC SEC. Comparative SDS-PAGE analysis showed similar purity to Protein A-purified Fc fusion protein. Host cell protein was quantified using HEK CHP ELISA, and product activity was confirmed via Cetuximab™ biosimilar binding in an ELISA format. https://images.squarespace-cdn.com/content/v1/6698031d5baeb54365a9a728/635b5a4b-e1d8-482b-8621-f4b16a03e4e7/f7.JPG Blog - Alternative Two Step Purification of a Soluble Receptor Fc Fusion Biologic Using Mixed-Mode Chromatography  - Make it stand out Fig. 7 HPLC SEC of the Captured EGFR:Fc and Polished Product on HPQ (Bio-Rad) https://images.squarespace-cdn.com/content/v1/6698031d5baeb54365a9a728/122e1274-b92c-4ef1-98f9-6d001f635e4f/t3.JPG Blog - Alternative Two Step Purification of a Soluble Receptor Fc Fusion Biologic Using Mixed-Mode Chromatography  - Make it stand out Table 2 Overall yield was over 70%. Final aggregate was below 1% by HPLC SEC. Clearance of HCP protein and residual DNA were comparable to Protein A.. Binding of the Fc fusion protein was retained against the common biologic antibody Cetuximab. https://www.picelkem.com/blog/concept-to-data-beginner-friendly-guide-to-starting-your-first-biologic-investigation monthly 0.5 2026-04-19 https://www.picelkem.com/blog/producing-human-il11-cytokine-for-pulmonary-fibrosis-studies-wgc5r monthly 0.5 2025-11-12 https://images.squarespace-cdn.com/content/v1/6698031d5baeb54365a9a728/3a2a8718-a10d-4a0a-b9ec-1043ccc4d394/T1.JPG Blog - Mixed-modal Ligand in the Capture of Rat and Mouse Antibodies - Make it stand out Table 1. Dynamic binding capacity model antibodies. https://images.squarespace-cdn.com/content/v1/6698031d5baeb54365a9a728/bc512ca5-3c47-44f0-8208-15823bebd7d6/T2.JPG Blog - Mixed-modal Ligand in the Capture of Rat and Mouse Antibodies - Make it stand out Table 2 Capture step hybridoma production media on 4-MEP and Capto MMC. https://images.squarespace-cdn.com/content/v1/6698031d5baeb54365a9a728/9bf0464d-8521-4182-b633-2b9f7746af94/T3.JPG Blog - Mixed-modal Ligand in the Capture of Rat and Mouse Antibodies - Make it stand out Table 3. Three step purification of hybridoma production media on 4-MEP, Capto MMC and Protein G coupled resin. https://images.squarespace-cdn.com/content/v1/6698031d5baeb54365a9a728/89e90f8a-15fa-4fbc-8912-87ab15642979/F1.JPG Blog - Mixed-modal Ligand in the Capture of Rat and Mouse Antibodies - Make it stand out Fig. 1. Commonly used elution buffers for Protein G on three model rodent antibodies. Antibody was bound at 3 mg/mL resin at 5 min residence time then quickly neutralized upon elution. https://images.squarespace-cdn.com/content/v1/6698031d5baeb54365a9a728/b53c8d81-7cfc-4fe3-97d6-140b41b5ec6e/F2.JPG Blog - Mixed-modal Ligand in the Capture of Rat and Mouse Antibodies - Make it stand out Fig. 2. Denaturation-induced aggregates from the Protein G elution step by UPLC Analytical SEC Assay. The amount of aggregation increases with low pH and increased acid strength. https://images.squarespace-cdn.com/content/v1/6698031d5baeb54365a9a728/9e640d88-0be2-4c94-ad9c-36a0139d706f/F3.JPG Blog - Mixed-modal Ligand in the Capture of Rat and Mouse Antibodies - Make it stand out Fig. 3. Structures of A) mercapto-ethyl-pyridine (4-MEP) a strong anion exchanger and B) Nbenzoyl-homocysteine (Capto MMC) a weak cation exchanger. https://images.squarespace-cdn.com/content/v1/6698031d5baeb54365a9a728/b6a49a67-45a1-4377-aa96-70d8133e13c8/F4.JPG Blog - Mixed-modal Ligand in the Capture of Rat and Mouse Antibodies - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/6698031d5baeb54365a9a728/8815cfca-f51d-4c1f-8b10-fa25e086b1be/F5.JPG Blog - Mixed-modal Ligand in the Capture of Rat and Mouse Antibodies - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://www.picelkem.com/blog/producing-human-il11-cytokine-for-pulmonary-fibrosis-studies monthly 0.5 2025-11-07 https://images.squarespace-cdn.com/content/v1/6698031d5baeb54365a9a728/be2389ae-288c-48e7-b4b0-61afc6317371/pi+fig+1.png Blog - Undergraduate Research Spotlight: Producing Human 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